CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

April 10th, 2017 by Mingzi M Zhang

Nature Chemical Biology 13, 607 (2017). doi:10.1038/nchembio.2341

Authors: Mingzi M Zhang, Fong Tian Wong, Yajie Wang, Shangwen Luo, Yee Hwee Lim, Elena Heng, Wan Lin Yeo, Ryan E Cobb, Behnam Enghiad, Ee Lui Ang & Huimin Zhao

Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.

  • Posted in Nat Chem Biol, Publications
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Measurement of In Vivo Protein Binding Affinities in a Signaling Network with Mass Spectrometry

April 5th, 2017 by Mumun Gencoglu, Alexander Schmidt and Attila Becskei

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.6b00282

AtaT blocks translation initiation by N-acetylation of the initiator tRNAfMet

April 3rd, 2017 by Dukas Jurėnas

Nature Chemical Biology 13, 640 (2017). doi:10.1038/nchembio.2346

Authors: Dukas Jurėnas, Sneha Chatterjee, Albert Konijnenberg, Frank Sobott, Louis Droogmans, Abel Garcia-Pino & Laurence Van Melderen

  • Posted in Nat Chem Biol, Publications
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Phosphorylated glycosphingolipids essential for cholesterol mobilization in Caenorhabditis elegans

April 3rd, 2017 by Sebastian Boland

Nature Chemical Biology 13, 647 (2017). doi:10.1038/nchembio.2347

Authors: Sebastian Boland, Ulrike Schmidt, Vyacheslav Zagoriy, Julio L Sampaio, Raphael F Fritsche, Regina Czerwonka, Tilo Lübken, Jakob Reimann, Sider Penkov, Hans-Joachim Knölker & Teymuras V Kurzchalia

  • Posted in Nat Chem Biol, Publications
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A Glucose-Sensing Toggle Switch for Autonomous, High Productivity Genetic Control

March 30th, 2017 by William Bothfeld, Grace Kapov and Keith E. J. Tyo

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.6b00257

The Role of SufS Is Restricted to Fe–S Cluster Biosynthesis in Escherichia coli

March 30th, 2017 by Martin Bühning, Angelo Valleriani and Silke Leimkühler

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Biochemistry
DOI: 10.1021/acs.biochem.7b00040

Simultaneous Measurement of Glucose-6-phosphate 3-Dehydrogenase (NtdC) Catalysis and the Nonenzymatic Reaction of Its Product: Kinetics and Isotope Effects on the First Step in Kanosamine Biosynthesis

March 29th, 2017 by Natasha D. Vetter and David R. J. Palmer

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Biochemistry
DOI: 10.1021/acs.biochem.7b00079

Structural and functional insight into human O-GlcNAcase

March 27th, 2017 by Christian Roth

Nature Chemical Biology 13, 610 (2017). doi:10.1038/nchembio.2358

Authors: Christian Roth, Sherry Chan, Wendy A Offen, Glyn R Hemsworth, Lianne I Willems, Dustin T King, Vimal Varghese, Robert Britton, David J Vocadlo & Gideon J Davies

O-GlcNAc hydrolase (OGA) removes O-linked N-acetylglucosamine (O-GlcNAc) from a myriad of nucleocytoplasmic proteins. Through co-expression and assembly of OGA fragments, we determined the three-dimensional structure of human OGA, revealing an unusual helix-exchanged dimer that lays a structural foundation for an improved understanding of substrate recognition and regulation of OGA. Structures of OGA in complex with a series of inhibitors define a precise blueprint for the design of inhibitors that have clinical value.

Near-infrared optogenetic pair for protein regulation and spectral multiplexing

March 27th, 2017 by Taras A Redchuk

Nature Chemical Biology 13, 633 (2017). doi:10.1038/nchembio.2343

Authors: Taras A Redchuk, Evgeniya S Omelina, Konstantin G Chernov & Vladislav V Verkhusha

  • Posted in Nat Chem Biol, Publications
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Chemical screening identifies ATM as a target for alleviating senescence

March 27th, 2017 by Hyun Tae Kang

Nature Chemical Biology 13, 616 (2017). doi:10.1038/nchembio.2342

Authors: Hyun Tae Kang, Joon Tae Park, Kobong Choi, Yongsub Kim, Hyo Jei Claudia Choi, Chul Won Jung, Young-Sam Lee & Sang Chul Park

  • Posted in Nat Chem Biol, Publications
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