Targeted protein degradation: You can glue it too!

April 13th, 2017 by Michal J Walczak

Nature Chemical Biology 13, 452 (2017). doi:10.1038/nchembio.2355

Authors: Michal J Walczak, Georg Petzold & Nicolas H Thomä

Proteolysis-targeting chimera (PROTACs) are synthetic molecules that recruit neo-substrate proteins to a ubiquitin ligase for ubiquitination and subsequent degradation. Structural insight into the VHL–MZ1–BRD4 complex reveals how the rationally designed MZ1–PROTAC molecule mediates degradation of an unnatural protein substrate.

Intrinsic Disorder: Fuzzy fast feedback

April 13th, 2017 by Mirella Bucci

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2367

Author: Mirella Bucci

Microbiology: A new language for small talk

April 13th, 2017 by Yi-Ming Shi

Nature Chemical Biology 13, 453 (2017). doi:10.1038/nchembio.2362

Authors: Yi-Ming Shi & Helge B Bode

A new signal–receptor pair involved in regulating biofilm formation and virulence was detected in Vibrio cholerae. Both the signal and the transcription factor belong to common classes of natural products and receptor proteins, suggesting widespread importance of related systems in nature.

Bacterial Membranes: Tear down this wall

April 13th, 2017 by Caitlin Deane

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2366

Author: Caitlin Deane

Optogenetics: Lighting up kinases

April 13th, 2017 by Grant Miura

Nature Chemical Biology 13, 451 (2017). doi:10.1038/nchembio.2365

Author: Grant Miura

Mechanistic insights into energy conservation by flavin-based electron bifurcation

April 10th, 2017 by Carolyn E Lubner

Nature Chemical Biology 13, 655 (2017). doi:10.1038/nchembio.2348

Authors: Carolyn E Lubner, David P Jennings, David W Mulder, Gerrit J Schut, Oleg A Zadvornyy, John P Hoben, Monika Tokmina-Lukaszewska, Luke Berry, Diep M Nguyen, Gina L Lipscomb, Brian Bothner, Anne K Jones, Anne-Frances Miller, Paul W King, Michael W W Adams & John W Peters

  • Posted in Nat Chem Biol, Publications
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CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

April 10th, 2017 by Mingzi M Zhang

Nature Chemical Biology 13, 607 (2017). doi:10.1038/nchembio.2341

Authors: Mingzi M Zhang, Fong Tian Wong, Yajie Wang, Shangwen Luo, Yee Hwee Lim, Elena Heng, Wan Lin Yeo, Ryan E Cobb, Behnam Enghiad, Ee Lui Ang & Huimin Zhao

Here we report an efficient CRISPR–Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.

  • Posted in Nat Chem Biol, Publications
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Measurement of In Vivo Protein Binding Affinities in a Signaling Network with Mass Spectrometry

April 5th, 2017 by Mumun Gencoglu, Alexander Schmidt and Attila Becskei

TOC Graphic

ACS Synthetic Biology
DOI: 10.1021/acssynbio.6b00282

AtaT blocks translation initiation by N-acetylation of the initiator tRNAfMet

April 3rd, 2017 by Dukas Jurėnas

Nature Chemical Biology 13, 640 (2017). doi:10.1038/nchembio.2346

Authors: Dukas Jurėnas, Sneha Chatterjee, Albert Konijnenberg, Frank Sobott, Louis Droogmans, Abel Garcia-Pino & Laurence Van Melderen

  • Posted in Nat Chem Biol, Publications
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Phosphorylated glycosphingolipids essential for cholesterol mobilization in Caenorhabditis elegans

April 3rd, 2017 by Sebastian Boland

Nature Chemical Biology 13, 647 (2017). doi:10.1038/nchembio.2347

Authors: Sebastian Boland, Ulrike Schmidt, Vyacheslav Zagoriy, Julio L Sampaio, Raphael F Fritsche, Regina Czerwonka, Tilo Lübken, Jakob Reimann, Sider Penkov, Hans-Joachim Knölker & Teymuras V Kurzchalia

  • Posted in Nat Chem Biol, Publications
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