Nucleic acids: mRNAs get a TREAT

November 21st, 2017 by Grant Miura

Nucleic acids: mRNAs get a TREAT

Nucleic acids: mRNAs get a TREAT, Published online: 21 November 2017; doi:10.1038/nchembio.2522

Nucleic acids: mRNAs get a TREAT

Genetic code expansion: Synthetases pick up the PACE

November 21st, 2017 by Jeffery M Tharp

Genetic code expansion: Synthetases pick up the PACE

Genetic code expansion: Synthetases pick up the PACE, Published online: 21 November 2017; doi:10.1038/nchembio.2516

Phage-assisted evolution can rapidly improve the efficiency and substrate specificity of orthogonal aminoacyl-tRNA synthetases. Furthermore, the crystal structure of the pyrrolysyl-tRNA synthetase N-terminal domain reveals the basis for these improvements and provides a structural rationale for orthogonality.

Errata: Functional annotation of chemical libraries across diverse biological processes

November 21st, 2017 by Jeff S Piotrowski

Errata: Functional annotation of chemical libraries across diverse biological processes

Errata: Functional annotation of chemical libraries across diverse biological processes, Published online: 21 November 2017; doi:10.1038/nchembio1217-1286b

Errata: Functional annotation of chemical libraries across diverse biological processes
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Errata: Functional annotation of chemical libraries across diverse biological processes

Errata: Functional annotation of chemical libraries across diverse biological processes

November 21st, 2017 by Jeff S Piotrowski

Errata: Functional annotation of chemical libraries across diverse biological processes

Errata: Functional annotation of chemical libraries across diverse biological processes, Published online: 21 November 2017; doi:10.1038/nchembio1217-1286a

Errata: Functional annotation of chemical libraries across diverse biological processes
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Errata: Functional annotation of chemical libraries across diverse biological processes

Peptide design: Hacking hemagglutinin

November 21st, 2017 by Karin Kuehnel

Peptide design: Hacking hemagglutinin

Peptide design: Hacking hemagglutinin, Published online: 21 November 2017; doi:10.1038/nchembio.2523

Peptide design: Hacking hemagglutinin

Yield Improvement of the Anti-MRSA Antibiotics WAP-8294A by CRISPR/dCas9 Combined with Refactoring Self-Protection Genes in Lysobacter enzymogenes OH11

November 20th, 2017 by Lingjun Yu, Wei Su, Paul D. Fey, Fengquan Liu and Liangcheng Du

TOC Graphic

ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00293
  • Posted in ACS Synthetic Biology, Publications
  • Comments Off on Yield Improvement of the Anti-MRSA Antibiotics WAP-8294A by CRISPR/dCas9 Combined with Refactoring Self-Protection Genes in Lysobacter enzymogenes OH11

Small-molecule inhibition of TLR8 through stabilization of its resting state

November 20th, 2017 by Shuting Zhang

Small-molecule inhibition of TLR8 through stabilization of its resting state

Small-molecule inhibition of TLR8 through stabilization of its resting state, Published online: 20 November 2017; doi:10.1038/nchembio.2518

High-throughput screening identified potent small-molecule inhibitors of the endosomal Toll-like receptor TLR8 that stabilize the preformed TLR8 dimer in its resting state by binding to a unique site on the inactive dimer interface.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Small-molecule inhibition of TLR8 through stabilization of its resting state

Engineering peptide ligase specificity by proteomic identification of ligation sites

November 20th, 2017 by Amy M Weeks

Engineering peptide ligase specificity by proteomic identification of ligation sites

Engineering peptide ligase specificity by proteomic identification of ligation sites, Published online: 20 November 2017; doi:10.1038/nchembio.2521

A comprehensive characterization of peptide ligase specificity using proteome-derived peptide libraries enables the identification of 72 new subtiligases and their application to site-specific bioconjugation and sequencing of the cellular N terminome.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Engineering peptide ligase specificity by proteomic identification of ligation sites

Monobactam formation in sulfazecin by a nonribosomal peptide synthetase thioesterase

November 20th, 2017 by Ryan A Oliver

Monobactam formation in sulfazecin by a nonribosomal peptide synthetase thioesterase

Monobactam formation in sulfazecin by a nonribosomal peptide synthetase thioesterase, Published online: 20 November 2017; doi:10.1038/nchembio.2526

Biosynthesis of the antibiotic sulfazecin involves N-sulfonation in trans of the tripeptide intermediate before synthesis of the β-lactam ring by a noncanonical thioesterase domain, demonstrating a new enzymatic route to the azetidinone moiety.
  • Posted in Nat Chem Biol, Publications
  • Comments Off on Monobactam formation in sulfazecin by a nonribosomal peptide synthetase thioesterase

Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing

November 14th, 2017 by Danielle N. Gallagher and James E. Haber

TOC Graphic

ACS Chemical Biology
DOI: 10.1021/acschembio.7b00760