[ASAP] Single-Molecule Localization Microscopy with the Fluorescence-Activating and Absorption-Shifting Tag (FAST) System
May 22nd, 2019 by ElizabethnM. Smith, Arnaud Gautier, Elias M. Puchner
[ASAP] Genetic Compatibility and Extensibility of Orthogonal Replication
May 21st, 2019 by Alex A. Javanpour, Chang C. Liu
Small-molecule control of antibody N-glycosylation in engineered mammalian cells
May 20th, 2019 by Michelle M. Chang
Nature Chemical Biology, Published online: 20 May 2019; doi:10.1038/s41589-019-0288-4
Synthetic gene circuits regulated by small molecules have been used to fine-tune glycosyltransferase expression in CHO cells, providing a method to produce therapeutic monoclonal antibodies with precise glycosylation states.Rewiring bacterial two-component systems by modular DNA-binding domain swapping
May 20th, 2019 by Sebastian R. Schmidl
Nature Chemical Biology, Published online: 20 May 2019; doi:10.1038/s41589-019-0286-6
Rewiring of bacterial two-component systems (TCSs) was achieved by DNA-binding domain swapping of the two largest response regulator families, which enables cross-species porting and provides a tool for identifying ligands for uncharacterized TCSs.Structural and mechanistic basis of mammalian Nudt12 RNA deNADding
May 17th, 2019 by Ewa Grudzien-Nogalska
Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0293-7
Structural and biochemical analyses identify Nudt12 as a novel mRNA deNADing enzyme in mammalian cells, which has a different substrate preference compared to the deNADing enzyme DXO.Covalent binding of uracil DNA glycosylase UdgX to abasic DNA upon uracil excision
May 17th, 2019 by Woo-Chan Ahn
Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0289-3
Structural analysis of uracil DNA glycosylases in complex with DNA reveals that conserved H109 acts as a nucleophile to attack the oxocarbenium ion and makes a covalent bond to the abasic site after uracil excision to form a stable intermediate.Arrestin-g pain
May 17th, 2019 by Mirella Bucci
Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0300-z
Arrestin-g painThe new face of nanowires
May 17th, 2019 by Caitlin Deane
Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0299-1
The new face of nanowiresSuicide inactivation of the uracil DNA glycosylase UdgX by covalent complex formation
May 17th, 2019 by Jie Tu
Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0290-x
Structural analysis of uracil DNA glycosylase reveals that its high affinity with DNA substrates derives from a stable intermediate that is formed by conservative H109 in a protruding loop covalently binding to the abasic site after uracil is excised.Natural fumigation as a mechanism for volatile transport between flower organs
May 17th, 2019 by Benoît Boachon
Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0287-5
Bioactive sesquiterpenes accumulating in petunia stigmas are synthesized in the floral tube and then transported to the pistil via natural fumigation within the internal airspace of the developing flower.