A PCBP1–BolA2 chaperone complex delivers iron for cytosolic [2Fe–2S] cluster assembly

August 12th, 2019 by Sarju J. Patel

Nature Chemical Biology, Published online: 12 August 2019; doi:10.1038/s41589-019-0330-6

The iron chaperone poly(rC)-binding protein 1 (PCBP1) coordinates ferrous iron via its KH3 domain and, together with BolA2 and glutathione, forms a complex that is required for the assembly of [2Fe–2S] clusters on the cytosolic BolA2–Glrx3 chaperone.
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A split CRISPR–Cpf1 platform for inducible genome editing and gene activation

August 12th, 2019 by Yuta Nihongaki

Nature Chemical Biology, Published online: 12 August 2019; doi:10.1038/s41589-019-0338-y

Split Cpf1 pairs are identified to enable chemical- and light-induced genome editing via dimerization. Another pair of split Cpf1 can be used to activate gene expression with high efficiency in cells and in mice.
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Inducible asymmetric cell division and cell differentiation in a bacterium

August 12th, 2019 by Nikolai V. Mushnikov

Nature Chemical Biology, Published online: 12 August 2019; doi:10.1038/s41589-019-0340-4

Small molecule or light-inducible gene circuits in Escherichia coli enable asymmetric cell pole localization of diguanylate phosphodiesterase and facilitate asymmetric cell division regulated by c-di-GMP-responsive transcription factors.
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Convergent biosynthetic transformations to a bacterial specialized metabolite

August 12th, 2019 by Yi-Ling Du

Nature Chemical Biology, Published online: 12 August 2019; doi:10.1038/s41589-019-0331-5

The indolmycin biosynthetic pathway in a marine gram-negative bacterium is distinct from its counterpart in terrestrial gram-positive Streptomyces species, using a Streptomyces shunt product as a substrate for an N-demethylindolmycin synthase.
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Structure and chemistry of lysinoalanine crosslinking in the spirochaete flagella hook

August 12th, 2019 by Michael J. Lynch

Nature Chemical Biology, Published online: 12 August 2019; doi:10.1038/s41589-019-0341-3

Structural and biochemical characterization of the spirochaete flagellar hook protein FlgE reveals how cysteine and lysine residues spontaneously react to form an interdomain lysinoalanine crosslink without the involvement of additional enzymes.
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A synthetic system for asymmetric cell division in <i>Escherichia coli</i>

August 12th, 2019 by Sara Molinari

Nature Chemical Biology, Published online: 12 August 2019; doi:10.1038/s41589-019-0339-x

The chromosomal partitioning system (par) of Caulobacter crescentus was repurposed to create an inducible genetic circuit for asymmetric plasmid partitioning and cell division in Escherichia coli.
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[ASAP] Construction of Genetic Logic Gates Based on the T7 RNA Polymerase Expression System in <italic toggle=”yes”>Rhodococcus opacus</italic> PD630

August 6th, 2019 by Drew M. DeLorenzo† and Tae Seok Moon*†‡

TOC Graphic

ACS Synthetic Biology
DOI: 10.1021/acssynbio.9b00213
  • Posted in ACS Synthetic Biology, Publications
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2-Hydroxyacyl-CoA lyase catalyzes acyloin condensation for one-carbon bioconversion

August 5th, 2019 by Alexander Chou

Nature Chemical Biology, Published online: 05 August 2019; doi:10.1038/s41589-019-0328-0

A bacterial 2-hydroxyacyl-CoA lyase catalyzes ligation of carbonyl-containing molecules of different chain lengths with formyl-CoA to produce elongated 2-hydroxyacyl-CoAs, enabling a one-carbon bioconversion pathway with formaldehyde as a substrate.
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Sweetly profiling the cysteinome

August 5th, 2019 by Jing Yang

Nature Chemical Biology, Published online: 05 August 2019; doi:10.1038/s41589-019-0348-9

A new sugar-based cysteine-reactive probe, combined with competitive activity-based protein profiling (ABPP), enables site-centric target deconvolution of itaconate in native proteomes, shedding light on a novel mechanism of action for this important immunoregulatory metabolite in inflammatory macrophages.

Site-specific m<sup>6</sup>A editing

August 5th, 2019 by Jiangbo Wei

Nature Chemical Biology, Published online: 05 August 2019; doi:10.1038/s41589-019-0349-8

The N6-methyladenosine modification on RNA affects almost all steps of RNA metabolism. A new approach, using the CRISPR-based technology to modulate m6A level in mRNA, enables direct functional interrogation of site-specific m6A.