Atomic structure of potato virus X, the prototype of the <i>Alphaflexiviridae</i> family
March 16th, 2020 by Alessandro Grinzato
Nature Chemical Biology, Published online: 16 March 2020; doi:10.1038/s41589-020-0502-4
A cryo-EM structure of potato virus X shows that virions are formed by repeats of segments of coat protein protomers arranged in a left-handed helical structure and reveals details of the RNA–capsid interactions, which shed light on virus assembly.<span class=”small-caps”>d</span>-Cycloserine destruction by alanine racemase and the limit of irreversible inhibition
March 16th, 2020 by Cesira de Chiara
Nature Chemical Biology, Published online: 16 March 2020; doi:10.1038/s41589-020-0498-9
d-Cycloserine inactivates alanine racemase by forming an adduct with the pyridoxal 5′-phosphate cofactor, but structural and spectroscopic analyses reveal that reactivation occurs on adduct hydrolysis and product rearrangement to a stable oxime.[ASAP] Yeast Surface Display System for Facilitated Production and Application of Phage Endolysin
March 10th, 2020 by
[ASAP] The O-GlcNAc Modification on Kinases
March 9th, 2020 by Paul A. Schwein and Christina M. Woo*
A bifunctional O-antigen polymerase structure reveals a new glycosyltransferase family
March 9th, 2020 by Bradley R. Clarke
Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0494-0
Structural characterization of WbbM, an enzyme involved in O2a-antigen biosynthesis in Klebsiella pneumoniae, reveals two unique active sites with galactopyranosyl- or galactofuranosyl-transferase activities for oligosaccharide polymerization.Activation of the α<sub>2B</sub> adrenoceptor by the sedative sympatholytic dexmedetomidine
March 9th, 2020 by Daopeng Yuan
Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0492-2
A cryo-EM structure of the GPCR α2B adrenergic receptor (α2BAR) in complex with the selective agonist dexmedetomidine and the G protein Go suggests a mechanism of selective activation and provides insights into G-protein coupling activity.A gene signal amplifier platform for monitoring the unfolded protein response
March 9th, 2020 by Carlos A. Origel Marmolejo
Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0497-x
Engineering of a synthetic circuit that couples transcriptional and protein turnover regulation to a fluorescent protein readout enables sensitive monitoring and amplification of gene expression signals from the unfolded protein response pathway.Deep mutational scanning reveals the structural basis for α-synuclein activity
March 9th, 2020 by Robert W. Newberry
Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0480-6
Deep mutational scanning reveals that α-synuclein adopts a membrane-bound α-helix conformation with increasing dynamics towards the C terminus. This helical conformation is associated with its cytotoxicity.A widespread role for SLC transmembrane transporters in resistance to cytotoxic drugs
March 9th, 2020 by Enrico Girardi
Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0483-3
A set of CRISPR–Cas9-based genetic screens in a haploid human cell line identifies more than 200 gene–drug associations involving solute carriers (SLCs), transporters important for the uptake and activity of cytotoxic drugs.Hitchhiking into the cell
March 9th, 2020 by Douglas B. Kell
Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0489-x
The mechanisms by which bioactive drugs get into their target cells is a question that has been greatly neglected. A new survey with CRISPR–Cas9 shows the widespread importance of protein transporters called solute carriers (SLCs).