CRISPR-suppressor scanning reveals a nonenzymatic role of LSD1 in AML

April 15th, 2019 by Michael E. Vinyard

CRISPR-suppressor scanning reveals a nonenzymatic role of LSD1 in AML

CRISPR-suppressor scanning reveals a nonenzymatic role of LSD1 in AML, Published online: 15 April 2019; doi:10.1038/s41589-019-0263-0

A CRISPR–Cas9 screening-based structure–activity relationship profiling method reveals that LSD1 inhibitors suppress acute myeloid leukemia by disruption of the interaction between LSD1 and GFI1B instead of the enzyme activity of LSD1.
  • Posted in Nat Chem Biol, Publications
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Dynamic assembly of protein disulfide isomerase in catalysis of oxidative folding

April 15th, 2019 by Masaki Okumura

Dynamic assembly of protein disulfide isomerase in catalysis of oxidative folding

Dynamic assembly of protein disulfide isomerase in catalysis of oxidative folding, Published online: 15 April 2019; doi:10.1038/s41589-019-0268-8

Single-molecule analysis by high-speed atomic force microscopy reveals that oxidized protein disulfide isomerase adopts a dynamic conformation in the absence of substrates and forms face-to-face dimers to accelerate oxidative folding in the presence of substrates.
  • Posted in Nat Chem Biol, Publications
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ARGuing for a new kinase class

April 8th, 2019 by Titus J. Boggon

ARGuing for a new kinase class

ARGuing for a new kinase class, Published online: 08 April 2019; doi:10.1038/s41589-019-0272-z

Arginine protein kinases regulate bacterial signaling cascades through phosphorylation of key arginine residues. Crystal structures of the kinase McsB provide the first glimpse of how these unusual enzymes recognize their protein substrates and are allosterically regulated.

Structure of McsB, a protein kinase for regulated arginine phosphorylation

April 8th, 2019 by Marcin J. Suskiewicz

Structure of McsB, a protein kinase for regulated arginine phosphorylation

Structure of McsB, a protein kinase for regulated arginine phosphorylation, Published online: 08 April 2019; doi:10.1038/s41589-019-0265-y

Structural and biochemical characterization of arginine kinase McsB reveals how its phosphagen kinase–like catalytic domain is adapted to target protein substrates while its phosphoarginine-binding domain allosterically controls kinase activity.
  • Posted in Nat Chem Biol, Publications
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Bromodomain inhibition of the coactivators CBP/EP300 facilitate cellular reprogramming

April 8th, 2019 by Ayyub Ebrahimi

Bromodomain inhibition of the coactivators CBP/EP300 facilitate cellular reprogramming

Bromodomain inhibition of the coactivators CBP/EP300 facilitate cellular reprogramming, Published online: 08 April 2019; doi:10.1038/s41589-019-0264-z

A chromatin-focused chemical screen identified CBP/EP300 bromodomain inhibitors as enhancers of reprogramming. These inhibitors decrease histone H3 lysine 27 acetylation, chromatin accessibility and expression of somatic-specific genes.
  • Posted in Nat Chem Biol, Publications
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Author Correction: tRNA tracking for direct measurements of protein synthesis kinetics in live cells

April 5th, 2019 by Ivan L. Volkov

Author Correction: tRNA tracking for direct measurements of protein synthesis kinetics in live cells

Author Correction: tRNA tracking for direct measurements of protein synthesis kinetics in live cells, Published online: 05 April 2019; doi:10.1038/s41589-019-0266-x

Author Correction: tRNA tracking for direct measurements of protein synthesis kinetics in live cells
  • Posted in Nat Chem Biol, Publications
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[ASAP] Building an Inducible T7 RNA Polymerase/T7 Promoter Circuit in <italic toggle=”yes”>Synechocystis</italic> sp. PCC6803

April 2nd, 2019 by Haojie Jin, Peter Lindblad, Devaki Bhaya

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.8b00515
  • Posted in ACS Synthetic Biology, Publications
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[ASAP] Combinatorial Sensor Design in <italic toggle=”yes”>Caulobacter crescentus</italic> for Selective Environmental Uranium Detection

April 2nd, 2019 by Dan M. Park, Michael J. Taffet

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.8b00484
  • Posted in ACS Synthetic Biology, Publications
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[ASAP] Systematic Evaluation of Genetic and Environmental Factors Affecting Performance of Translational Riboswitches

April 1st, 2019 by R. Kent, N. Dixon

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.9b00017
  • Posted in ACS Synthetic Biology, Publications
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[ASAP] An Accessible Continuous-Culture Turbidostat for Pooled Analysis of Complex Libraries

April 1st, 2019 by Anna M. McGeachy, Zuriah A. Meacham, Nicholas T. Ingolia

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.8b00529