[ASAP] Single-Molecule Localization Microscopy with the Fluorescence-Activating and Absorption-Shifting Tag (FAST) System

May 22nd, 2019 by ElizabethnM. Smith, Arnaud Gautier, Elias M. Puchner

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ACS Chemical Biology
DOI: 10.1021/acschembio.9b00149
  • Posted in ACS Chemical Biology, Publications
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[ASAP] Genetic Compatibility and Extensibility of Orthogonal Replication

May 21st, 2019 by Alex A. Javanpour, Chang C. Liu

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ACS Synthetic Biology
DOI: 10.1021/acssynbio.9b00122

Small-molecule control of antibody N-glycosylation in engineered mammalian cells

May 20th, 2019 by Michelle M. Chang

Nature Chemical Biology, Published online: 20 May 2019; doi:10.1038/s41589-019-0288-4

Synthetic gene circuits regulated by small molecules have been used to fine-tune glycosyltransferase expression in CHO cells, providing a method to produce therapeutic monoclonal antibodies with precise glycosylation states.
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Rewiring bacterial two-component systems by modular DNA-binding domain swapping

May 20th, 2019 by Sebastian R. Schmidl

Nature Chemical Biology, Published online: 20 May 2019; doi:10.1038/s41589-019-0286-6

Rewiring of bacterial two-component systems (TCSs) was achieved by DNA-binding domain swapping of the two largest response regulator families, which enables cross-species porting and provides a tool for identifying ligands for uncharacterized TCSs.
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Structural and mechanistic basis of mammalian Nudt12 RNA deNADding

May 17th, 2019 by Ewa Grudzien-Nogalska

Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0293-7

Structural and biochemical analyses identify Nudt12 as a novel mRNA deNADing enzyme in mammalian cells, which has a different substrate preference compared to the deNADing enzyme DXO.

Covalent binding of uracil DNA glycosylase UdgX to abasic DNA upon uracil excision

May 17th, 2019 by Woo-Chan Ahn

Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0289-3

Structural analysis of uracil DNA glycosylases in complex with DNA reveals that conserved H109 acts as a nucleophile to attack the oxocarbenium ion and makes a covalent bond to the abasic site after uracil excision to form a stable intermediate.
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Arrestin-g pain

May 17th, 2019 by Mirella Bucci

Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0300-z

Arrestin-g pain

The new face of nanowires

May 17th, 2019 by Caitlin Deane

Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0299-1

The new face of nanowires

Suicide inactivation of the uracil DNA glycosylase UdgX by covalent complex formation

May 17th, 2019 by Jie Tu

Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0290-x

Structural analysis of uracil DNA glycosylase reveals that its high affinity with DNA substrates derives from a stable intermediate that is formed by conservative H109 in a protruding loop covalently binding to the abasic site after uracil is excised.
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Natural fumigation as a mechanism for volatile transport between flower organs

May 17th, 2019 by BenoƮt Boachon

Nature Chemical Biology, Published online: 17 May 2019; doi:10.1038/s41589-019-0287-5

Bioactive sesquiterpenes accumulating in petunia stigmas are synthesized in the floral tube and then transported to the pistil via natural fumigation within the internal airspace of the developing flower.
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