Atomic structure of potato virus X, the prototype of the <i>Alphaflexiviridae</i> family

March 16th, 2020 by Alessandro Grinzato

Nature Chemical Biology, Published online: 16 March 2020; doi:10.1038/s41589-020-0502-4

A cryo-EM structure of potato virus X shows that virions are formed by repeats of segments of coat protein protomers arranged in a left-handed helical structure and reveals details of the RNA–capsid interactions, which shed light on virus assembly.
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<span class=”small-caps”>d</span>-Cycloserine destruction by alanine racemase and the limit of irreversible inhibition

March 16th, 2020 by Cesira de Chiara

Nature Chemical Biology, Published online: 16 March 2020; doi:10.1038/s41589-020-0498-9

d-Cycloserine inactivates alanine racemase by forming an adduct with the pyridoxal 5′-phosphate cofactor, but structural and spectroscopic analyses reveal that reactivation occurs on adduct hydrolysis and product rearrangement to a stable oxime.
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[ASAP] Yeast Surface Display System for Facilitated Production and Application of Phage Endolysin

March 10th, 2020 by

TOC Graphic

ACS Synthetic Biology
DOI: 10.1021/acssynbio.9b00360

[ASAP] The O-GlcNAc Modification on Kinases

March 9th, 2020 by Paul A. Schwein and Christina M. Woo*

TOC Graphic

ACS Chemical Biology
DOI: 10.1021/acschembio.9b01015

A bifunctional O-antigen polymerase structure reveals a new glycosyltransferase family

March 9th, 2020 by Bradley R. Clarke

Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0494-0

Structural characterization of WbbM, an enzyme involved in O2a-antigen biosynthesis in Klebsiella pneumoniae, reveals two unique active sites with galactopyranosyl- or galactofuranosyl-transferase activities for oligosaccharide polymerization.
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Activation of the α<sub>2B</sub> adrenoceptor by the sedative sympatholytic dexmedetomidine

March 9th, 2020 by Daopeng Yuan

Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0492-2

A cryo-EM structure of the GPCR α2B adrenergic receptor (α2BAR) in complex with the selective agonist dexmedetomidine and the G protein Go suggests a mechanism of selective activation and provides insights into G-protein coupling activity.
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A gene signal amplifier platform for monitoring the unfolded protein response

March 9th, 2020 by Carlos A. Origel Marmolejo

Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0497-x

Engineering of a synthetic circuit that couples transcriptional and protein turnover regulation to a fluorescent protein readout enables sensitive monitoring and amplification of gene expression signals from the unfolded protein response pathway.
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Deep mutational scanning reveals the structural basis for α-synuclein activity

March 9th, 2020 by Robert W. Newberry

Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0480-6

Deep mutational scanning reveals that α-synuclein adopts a membrane-bound α-helix conformation with increasing dynamics towards the C terminus. This helical conformation is associated with its cytotoxicity.
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A widespread role for SLC transmembrane transporters in resistance to cytotoxic drugs

March 9th, 2020 by Enrico Girardi

Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0483-3

A set of CRISPR–Cas9-based genetic screens in a haploid human cell line identifies more than 200 gene–drug associations involving solute carriers (SLCs), transporters important for the uptake and activity of cytotoxic drugs.
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Hitchhiking into the cell

March 9th, 2020 by Douglas B. Kell

Nature Chemical Biology, Published online: 09 March 2020; doi:10.1038/s41589-020-0489-x

The mechanisms by which bioactive drugs get into their target cells is a question that has been greatly neglected. A new survey with CRISPR–Cas9 shows the widespread importance of protein transporters called solute carriers (SLCs).