Computational redesign of enzymes for regio- and enantioselective hydroamination

May 21st, 2018 by Ruifeng Li

Computational redesign of enzymes for regio- and enantioselective hydroamination

Computational redesign of enzymes for regio- and enantioselective hydroamination, Published online: 21 May 2018; doi:10.1038/s41589-018-0053-0

Computational protein design, without subsequent directed evolution, rapidly provides a set of aspartase variants capable of biocatalytic asymmetric addition of ammonia to substituted acrylates, producing various enantiopure β-amino acids.
  • Posted in Nat Chem Biol, Publications
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Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA

May 21st, 2018 by Xiaoting Shu

Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA

Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA, Published online: 21 May 2018; doi:10.1038/s41589-018-0065-9

A genome-wide uracil profiling technology (termed “dU-seq”), based on selective labeling and biotin pull-down, reveals that uracil is enriched at the centromere of the human genome.
  • Posted in Nat Chem Biol, Publications
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2018 Recipients of the Gordon Hammes Biochemistry Lecutureship and Scholar Awards have been Announced!

May 17th, 2018 by pthomas2

The recipient of the 2018 Gordon Hammes Lectureship is Vern L. Schramm, Albert Einstein College of Medicine and the recipient of the Gordon Hammes Scholar Award is Timothy Bumpus, Cornell University.  Read more at the <a href=”/awards/recipients”>award recipients</a> page.

Membrane association of monotopic phosphoglycosyl transferase underpins function

May 16th, 2018 by Leah C. Ray

Membrane association of monotopic phosphoglycosyl transferase underpins function

Membrane association of monotopic phosphoglycosyl transferase underpins function, Published online: 16 May 2018; doi:10.1038/s41589-018-0054-z

The structure of a monotopic polyprenol phosphate phosphoglycosyl transferase, PglC, reveals how it interacts with the bacterial membrane and coordinates a reaction between membrane-embedded and soluble substrates during glycoconjugate assembly.
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Electron handoff

May 16th, 2018 by Caitlin Deane

Electron handoff

Electron handoff, Published online: 16 May 2018; doi:10.1038/s41589-018-0073-9

Electron handoff

Buffering transition

May 16th, 2018 by Yiyun Song

Buffering transition

Buffering transition, Published online: 16 May 2018; doi:10.1038/s41589-018-0076-6

Buffering transition

Tracking tRNA packages

May 16th, 2018 by Achillefs N. Kapanidis

Tracking tRNA packages

Tracking tRNA packages, Published online: 16 May 2018; doi:10.1038/s41589-018-0066-8

Fast, single-molecule tracking microscopy monitors transitions between mobile and ribosome-bound fluorescent tRNAs to achieve nucleotide-resolution measurements of translation rates in living cells.

Functional phosphorylation

May 16th, 2018 by Mirella Bucci

Functional phosphorylation

Functional phosphorylation, Published online: 16 May 2018; doi:10.1038/s41589-018-0075-7

Functional phosphorylation

Identification of a <i>S. aureus</i> virulence factor by activity-based protein profiling (ABPP)

May 16th, 2018 by Christian S. Lentz

Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP)

Identification of a <i>S. aureus</i> virulence factor by activity-based protein profiling (ABPP), Published online: 16 May 2018; doi:10.1038/s41589-018-0060-1

ABP profiling identifies uncharacterized S. aureus serine hydrolases, including the surface-localized FphB, which processes lipid ester substrates and is required for infection in vivo. An FphB inhibitor reduces in vivo bacterial load.
  • Posted in Nat Chem Biol, Publications
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Next-generation biocontainment systems for engineered organisms

May 16th, 2018 by Jeong Wook Lee

Next-generation biocontainment systems for engineered organisms

Next-generation biocontainment systems for engineered organisms, Published online: 16 May 2018; doi:10.1038/s41589-018-0056-x

Synthetic biology offers innovative approaches for engineering biological systems, but also supports the development of biocontainment strategies that ensure the safe application of genetically modified organisms.