The splice is right

October 15th, 2018 by James Palacino

The splice is right

The splice is right, Published online: 15 October 2018; doi:10.1038/s41589-018-0147-8

Current drug discovery efforts focus on proteins because of their ability to form stable, structured pockets. A recent study demonstrates that targeting stable, structured bioactive RNA motifs, such as autocatalytic introns, may provide a novel method of expanding druggability and selectivity.

In vivo chloride concentrations surge to proteotoxic levels during acid stress

October 15th, 2018 by Frederick Stull

In vivo chloride concentrations surge to proteotoxic levels during acid stress

In vivo chloride concentrations surge to proteotoxic levels during acid stress, Published online: 15 October 2018; doi:10.1038/s41589-018-0143-z

Protonation of periplasmic protein carboxylic groups creates a Donnan equilibrium in the bacterial periplasmic space at low pH, leading to accumulation of Cl− and unfolding and aggregation of periplasmic proteins, which can be rescued by chaperones.
  • Posted in Nat Chem Biol, Publications
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Compartmentalizing acid stress in bacteria

October 15th, 2018 by Colin Kleanthous

Compartmentalizing acid stress in bacteria

Compartmentalizing acid stress in bacteria, Published online: 15 October 2018; doi:10.1038/s41589-018-0148-7

A Donnan equilibrium causes an influx of chloride ions into the Escherichia coli periplasm when the bacterium finds itself in gastric fluid. The combination of low pH and high anion concentration drives proteins to aggregate, a potentially lethal event unless prevented by specific chaperones.

[ASAP] Analysis of the Ub to Ub-CR Transition in Ubiquitin

October 14th, 2018 by Konstantin Röder, David J. Wales

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Biochemistry
DOI: 10.1021/acs.biochem.8b00770

[ASAP] High-Throughput Explorations of RNA Structural Modularity

October 14th, 2018 by Mark A. Boerneke, Kevin M. Weeks

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Biochemistry
DOI: 10.1021/acs.biochem.8b00999

[ASAP] Instantaneous Iodine-Assisted DNAzyme Cleavage of Phosphorothioate RNA

October 14th, 2018 by Po-Jung Jimmy Huang, Woohyun J. Moon, Juewen Liu

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Biochemistry
DOI: 10.1021/acs.biochem.8b00900

[ASAP] Crystal Structures of Wild-Type and F448A Mutant <italic toggle=”yes”>Citrobacter freundii</italic> Tyrosine Phenol-Lyase Complexed with a Substrate and Inhibitors: Implications for the Reaction Mechanism

October 11th, 2018 by Robert S. Phillips, Steven Craig

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Biochemistry
DOI: 10.1021/acs.biochem.8b00724

MCT2 mediates concentration-dependent inhibition of glutamine metabolism by MOG

October 8th, 2018 by Louise Fets

MCT2 mediates concentration-dependent inhibition of glutamine metabolism by MOG

MCT2 mediates concentration-dependent inhibition of glutamine metabolism by MOG, Published online: 08 October 2018; doi:10.1038/s41589-018-0136-y

High intracellular concentrations of the α-ketoglutarate analog N-oxalylglycine, owing to MCT2-mediated transport of its newly described prodrug MOG, inhibit multiple enzymes in glutamine metabolism and selectively kill MCT2-expressing cancer cells.
  • Posted in Nat Chem Biol, Publications
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New tricks for an old drug

October 8th, 2018 by Barbara S. Nelson

New tricks for an old drug

New tricks for an old drug, Published online: 08 October 2018; doi:10.1038/s41589-018-0137-x

The prodrug dimethyloxalylglycine (DMOG) is a well-known tool compound used to study hypoxia. New findings reveal that DMOG also inhibits glutamine metabolism and can be exploited to selectively kill some cancer cells, highlighting important caveats when using DMOG for hypoxia studies.

[ASAP] Characterization of Interactions and Phospholipid Transfer between Substrate Binding Proteins of the OmpC-Mla System

October 7th, 2018 by Bilge Ercan, Wen-Yi Low, Xuejun Liu, Shu-Sin Chng

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Biochemistry
DOI: 10.1021/acs.biochem.8b00897