Regulation of apoptosis by an intrinsically disordered region of Bcl-xL

March 5th, 2018 by Ariele Viacava Follis

Regulation of apoptosis by an intrinsically disordered region of Bcl-xL

Regulation of apoptosis by an intrinsically disordered region of Bcl-xL, Published online: 05 March 2018; doi:10.1038/s41589-018-0011-x

Post-translational modification of residues in an intrinsically disordered region of Bcl-XL promotes interactions with its folded core and allosterically reduces affinity for proapoptotic BH3-domain-containing proteins, resulting in apoptosis.
  • Posted in Nat Chem Biol, Publications
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A lanthipeptide library used to identify a protein–protein interaction inhibitor

March 5th, 2018 by Xiao Yang

A lanthipeptide library used to identify a protein–protein interaction inhibitor

A lanthipeptide library used to identify a protein–protein interaction inhibitor, Published online: 05 March 2018; doi:10.1038/s41589-018-0008-5

The substrate-tolerant lanthipeptide synthetase ProcM enables the construction of a plasmid-encoded library of bicyclic lanthipeptides, from which an inhibitor of the p6–UEV protein–protein interaction is identified by a reverse two-hybrid screen.
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Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands

March 5th, 2018 by Lara N. Gechijian

Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands

Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands, Published online: 05 March 2018; doi:10.1038/s41589-018-0010-y

Selective TRIM24 degradation is achieved by co-opting the VHL E3 ubiquitin ligase machinery. TRIM24 degradation outperforms bromodomain inhibition, with an enhanced antiproliferative effect in acute leukemia, a novel context of TRIM24 dependency.
  • Posted in Nat Chem Biol, Publications
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Chaperones convert the energy from ATP into the nonequilibrium stabilization of native proteins

March 5th, 2018 by Pierre Goloubinoff

Chaperones convert the energy from ATP into the nonequilibrium stabilization of native proteins

Chaperones convert the energy from ATP into the nonequilibrium stabilization of native proteins, Published online: 05 March 2018; doi:10.1038/s41589-018-0013-8

A nonequilibrium thermodynamic model can explain how molecular chaperones such as GroEL can use the energy from ATP hydrolysis to maintain substrate proteins in an active state, even under conditions that favor the substrate’s inactive unfolded state.
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Wicket: A Versatile Tool for the Integration and Optimization of Exogenous Pathways in Saccharomyces cerevisiae

February 28th, 2018 by Sha Hou, Qin Qin and Junbiao Dai

TOC Graphic

ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00391
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Directed Evolution of Membrane Transport Using Synthetic Selections

February 28th, 2018 by Anne P. Bali, Hans J. Genee and Morten O. A. Sommer

TOC Graphic

ACS Synthetic Biology
DOI: 10.1021/acssynbio.7b00407

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

February 26th, 2018 by Kiryl D. Piatkevich

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters, Published online: 26 February 2018; doi:10.1038/s41589-018-0004-9

Directed evolution of opsins via robotic high-content screening finds a fluorescent reporter of voltage that is simultaneously optimized for brightness, localization and voltage sensitivity and is applicable in three model systems.
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Resistance to nonribosomal peptide antibiotics mediated by <span class=”small-caps”>d</span>-stereospecific peptidases

February 26th, 2018 by Yong-Xin Li

Resistance to nonribosomal peptide antibiotics mediated by d-stereospecific peptidases

Resistance to nonribosomal peptide antibiotics mediated by <span class="small-caps">d</span>-stereospecific peptidases, Published online: 26 February 2018; doi:10.1038/s41589-018-0009-4

Networking-associated genome mining on bacterial genomes followed by chemical and enzymatic analysis identified a mechanism of resistance toward nonribosomal peptide antibiotics based on hydrolytic cleavage by d-stereospecific peptidases.
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Directed evolution of a synthetic phylogeny of programmable Trp repressors

February 26th, 2018 by Jared W. Ellefson

Directed evolution of a synthetic phylogeny of programmable Trp repressors

Directed evolution of a synthetic phylogeny of programmable Trp repressors, Published online: 26 February 2018; doi:10.1038/s41589-018-0006-7

Directed evolution of Trp repressor (TrpR) variants that are responsive to halogenated tryptophan analogs and recognize new operator sites serve as useful components for constructing complex gene expression networks.
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Trp-ing upon new repressors

February 26th, 2018 by Andreas K. Brödel

Trp-ing upon new repressors

Trp-ing upon new repressors, Published online: 26 February 2018; doi:10.1038/s41589-018-0012-9

Bioengineers have used directed evolution to generate a new family of synthetic transcription factors based on the tryptophan repressor. The evolved repressor family enables researchers to build new gene circuits for biomedical applications.