[ASAP] Genome, Epigenome, and Transcriptome Editing via Chemical Modification of Nucleobases in Living Cells

December 12th, 2018 by Brodie L. Ranzau, Alexis C. Komor

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Biochemistry
DOI: 10.1021/acs.biochem.8b00958

[ASAP] The Polycystin Complex Reveals Its Complexity

December 12th, 2018 by Valeria Padovano, Michael J. Caplan

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Biochemistry
DOI: 10.1021/acs.biochem.8b01205

Lanthanide lover

December 10th, 2018 by Caitlin Deane

Lanthanide lover

Lanthanide lover, Published online: 10 December 2018; doi:10.1038/s41589-018-0196-z

Lanthanide lover

Design of fast proteolysis-based signaling and logic circuits in mammalian cells

December 10th, 2018 by Tina Fink

Design of fast proteolysis-based signaling and logic circuits in mammalian cells

Design of fast proteolysis-based signaling and logic circuits in mammalian cells, Published online: 10 December 2018; doi:10.1038/s41589-018-0181-6

Protease-cleavable orthogonal-coiled-coil-based (SPOC) systems, in which split viral proteases are activated by small molecules and cleave coiled-coil protease substrates, reprogram signaling with rapid kinetics in mammalian cells.
  • Posted in Nat Chem Biol, Publications
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<i>N</i><sup>6-</sup>Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation

December 10th, 2018 by Honghui Ma

N6-Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation

<i>N</i><sup>6-</sup>Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation, Published online: 10 December 2018; doi:10.1038/s41589-018-0184-3

ZCCHC4 was identified as a mammalian ribosome RNA (rRNA) N6-methyladenosine (m6A) writer protein that installs m6A 4220 in 28S rRNA. 28S rRNA methylation affects global translation and cell growth and contributes to tumorigenesis in cancer cells.
  • Posted in Nat Chem Biol, Publications
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The bacteria that move me

December 10th, 2018 by Mirella Bucci

The bacteria that move me

The bacteria that move me, Published online: 10 December 2018; doi:10.1038/s41589-018-0198-x

The bacteria that move me

Riboregulated toehold-gated gRNA for programmable CRISPR–Cas9 function

December 10th, 2018 by Ka-Hei Siu

Riboregulated toehold-gated gRNA for programmable CRISPR–Cas9 function

Riboregulated toehold-gated gRNA for programmable CRISPR–Cas9 function, Published online: 10 December 2018; doi:10.1038/s41589-018-0186-1

Engineering of toehold-gated guide RNA (thgRNA) by tethering toehold riboswitches to sgRNAs enables the activation of CRISPR–Cas9 genome editing or transcriptional regulation in response to complementary synthetic or endogenous cellular RNAs.
  • Posted in Nat Chem Biol, Publications
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<i>N</i><sup>6</sup>-mA condenses chromatin

December 10th, 2018 by Yiyun Song

N6-mA condenses chromatin

<i>N</i><sup>6</sup>-mA condenses chromatin, Published online: 10 December 2018; doi:10.1038/s41589-018-0199-9

N6-mA condenses chromatin

Mechanistic insights revealed by a UBE2A mutation linked to intellectual disability

December 10th, 2018 by Juliana Ferreira de Oliveira

Mechanistic insights revealed by a UBE2A mutation linked to intellectual disability

Mechanistic insights revealed by a UBE2A mutation linked to intellectual disability, Published online: 10 December 2018; doi:10.1038/s41589-018-0177-2

Structural and biochemical analysis of a UBE2A mutation linked to intellectual disability reveals that the Q93E mutant perturbs the E2 catalytic microenvironment essential for lysine deprotonation during the ubiquitin-transfer process.
  • Posted in Nat Chem Biol, Publications
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E2 enzymes: lessons in ubiquitin transfer from XLID patients

December 10th, 2018 by Martina Foglizzo

E2 enzymes: lessons in ubiquitin transfer from XLID patients

E2 enzymes: lessons in ubiquitin transfer from XLID patients, Published online: 10 December 2018; doi:10.1038/s41589-018-0191-4

Characterization of a novel mutation in the E2 ubiquitin-conjugating enzyme UBE2A accounts for the decreased activity of the mutant enzyme that underlies disease and provides important insight into the catalytic mechanism of E2s.