A calcium-dependent acyltransferase that produces N-acyl phosphatidylethanolamines

July 11th, 2016 by Yuji Ogura

Nature Chemical Biology 12, 669 (2016). doi:10.1038/nchembio.2127

Authors: Yuji Ogura, William H Parsons, Siddhesh S Kamat & Benjamin F Cravatt

More than 30 years ago, a calcium-dependent enzyme activity was described that generates N-acyl phosphatidylethanolamines (NAPEs), which are precursors for N-acyl ethanolamine (NAE) lipid transmitters, including the endocannabinoid anandamide. The identity of this calcium-dependent N-acyltransferase (Ca-NAT) has remained mysterious. Here, we use activity-based protein profiling to identify the poorly characterized serine hydrolase PLA2G4E as a mouse brain Ca-NAT and show that this enzyme generates NAPEs and NAEs in mammalian cells.

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