A carbonate-forming Baeyer-Villiger monooxygenase

May 18th, 2014 by Youcai Hu

Nature Chemical Biology 10, 552 (2014). doi:10.1038/nchembio.1527

Authors: Youcai Hu, David Dietrich, Wei Xu, Ashay Patel, Justin A J Thuss, Jingjing Wang, Wen-Bing Yin, Kangjian Qiao, K N Houk, John C Vederas & Yi Tang

Despite the remarkable versatility displayed by flavin-dependent monooxygenases (FMOs) in natural product biosynthesis, one notably missing activity is the oxidative generation of carbonate functional groups. We describe a multifunctional Baeyer-Villiger monooxygenase, CcsB, which catalyzes the formation of an in-line carbonate in the macrocyclic portion of cytochalasin E. This study expands the repertoire of activities of FMOs and provides a possible synthetic strategy for transformation of ketones into carbonates.