Engineering nonphosphorylative metabolism to generate lignocellulose-derived products

February 8th, 2016 by Yi-Shu Tai

Nature Chemical Biology 12, 247 (2016). doi:10.1038/nchembio.2020

Authors: Yi-Shu Tai, Mingyong Xiong, Pooja Jambunathan, Jingyu Wang, Jilong Wang, Cole Stapleton & Kechun Zhang

  • Posted in Nat Chem Biol, Publications
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Enzyme pathways: C1 metabolism redesigned

May 15th, 2015 by Yi-Shu Tai

Nature Chemical Biology 11, 384 (2015). doi:10.1038/nchembio.1819

Authors: Yi-Shu Tai & Kechun Zhang

One-carbon metabolic pathways create new opportunities for metabolic engineering, but natural pathways have limitations in catalytic efficiency and interspecies transferability. Now a computationally designed enzyme, formolase, enables the construction of a synthetic metabolic pathway in Escherichia coli for assimilation of formate into a glycolytic intermediate in only five reaction steps.