Convergent biosynthetic transformations to a bacterial specialized metabolite

August 12th, 2019 by Yi-Ling Du

Nature Chemical Biology, Published online: 12 August 2019; doi:10.1038/s41589-019-0331-5

The indolmycin biosynthetic pathway in a marine gram-negative bacterium is distinct from its counterpart in terrestrial gram-positive Streptomyces species, using a Streptomyces shunt product as a substrate for an N-demethylindolmycin synthase.
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A heme-dependent enzyme forms the nitrogen–nitrogen bond in piperazate

June 19th, 2017 by Yi-Ling Du

Nature Chemical Biology 13, 836 (2017). doi:10.1038/nchembio.2411

Authors: Yi-Ling Du, Hai-Yan He, Melanie A Higgins & Katherine S Ryan

Molecules containing a nitrogen–nitrogen (N–N) linkage have a variety of structures and biological activities; however, no enzyme has yet been demonstrated to catalyze N–N bond formation in an organic molecule. Here we report that the heme-dependent enzyme KtzT from Kutzneria sp. 744 catalyzes N–N bond formation in the biosynthesis of piperazate, a building block for nonribosomal peptides.

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A pyridoxal phosphate–dependent enzyme that oxidizes an unactivated carbon-carbon bond

January 25th, 2016 by Yi-Ling Du

Nature Chemical Biology 12, 194 (2016). doi:10.1038/nchembio.2009

Authors: Yi-Ling Du, Rahul Singh, Lona M Alkhalaf, Eugene Kuatsjah, Hai-Yan He, Lindsay D Eltis & Katherine S Ryan

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