A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells

January 6th, 2020 by Tina Lebar

Nature Chemical Biology, Published online: 06 January 2020; doi:10.1038/s41589-019-0443-y

A set of orthogonal coiled-coil peptide heterodimers were developed to enable control of protein localization as well as transcriptional regulation by enhancing effector recruitment to TALE and CRISPR–dCas9 systems in mammalian cells and in vivo.
  • Posted in Nat Chem Biol, Publications
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Polarized displacement by transcription activator-like effectors for regulatory circuits

November 19th, 2018 by Tina Lebar

Polarized displacement by transcription activator-like effectors for regulatory circuits

Polarized displacement by transcription activator-like effectors for regulatory circuits, Published online: 19 November 2018; doi:10.1038/s41589-018-0163-8

The observation that transcription activator-like effectors (TALES) displace TALES bound at adjacent downstream DNA enables engineered regulation of gene expression, displacement of other DNA binding proteins and construction of logic-gated systems.
  • Posted in Nat Chem Biol, Publications
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