Post-translational site-selective protein backbone α-deuteration

September 17th, 2018 by Sébastien R. G. Galan

Post-translational site-selective protein backbone α-deuteration

Post-translational site-selective protein backbone α-deuteration, Published online: 17 September 2018; doi:10.1038/s41589-018-0128-y

A chemical method for site-selective deuterium exchange at protein backbone α-carbons, involving conversion of cysteine to dehydroalanine and then to deuterated cysteine, is used to explore the mechanism of a model protein bioconjugation reaction.