Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase
February 15th, 2019 by Maximilian Fottner
Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase
Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase, Published online: 15 February 2019; doi:10.1038/s41589-019-0227-4
A method combining genetic-code expansion, bioorthogonal Staudinger reduction and sortase-mediated transpeptidation enables site-specific and orthogonal modification of proteins with ubiquitin and SUMO in vitro and in living cells.