A Unique Tool for Cellular Structural Biology: In-cell NMR [Cell Biology]

December 16th, 2015 by Luchinat, E., Banci, L.

Conventional structural and chemical biology approaches are applied to macromolecules extrapolated from their native context. When doing so, important structural and functional features of macromolecules may be lost, which depend on their native network of interactions within the cell. In-cell nuclear magnetic resonance is a branch of biomolecular NMR spectroscopy that allows macromolecules to be analyzed in living cells, at the atomic level. In-cell NMR can be applied to several cellular systems to obtain biologically relevant structural and functional information. Here we summarize the existing approaches, and focus on the applications to protein folding, interactions and post-translational modifications.