Leucine-rich Repeat-containing G-protein Coupled Receptor 5 Marks Short-term Hematopoietic Stem and Progenitor Cells during Mouse Embryonic Development [Cell Biology]

June 25th, 2014 by Liu, D., He, X. C., Qian, P., Barker, N., Trainor, P. A., Clevers, H., Liu, H., Li, L.

Lgr5 is a marker for proliferating stem cells in adult intestine, stomach and hair follicle. However, Lgr5 is not expressed in adult hematopoietic stem and progenitor cells (HSPCs). Whether Lgr5 is expressed in the embryonic and fetal HSPCs that undergo rapid proliferation is unknown. Here we report the detection of Lgr5 expression in HSPCs in the aorta-gonad-mesonephros (AGM) and fetal liver. We also found that a portion of Lgr5+ cells expressed Runx-1 genes that are critical for the ontogeny of HSPCs. A small portion of Lgr5+ cells also expressed HSPC surface markers c-Kit and CD34 in AGM or CD41 in fetal liver. Furthermore, the majority of Lgr5+ cells expressed Ki67, indicating their proliferating state. Transplantation of fetal liver-derived Lgr5-GFP+ cells (E12.5) demonstrated that Lgr5-GFP+ cells were able to reconstitute myeloid and lymphoid lineages in adult recipients, but the engraftment was short-term (4-8 weeks) and 20-fold lower compared to the Lgr5-GFP- control. Our data show that Lgr5-expressing cells mark short-term hematopoietic stem and progenitor cells, consistent with the role of Lgr5 in supporting HSPCs rapid proliferation during embryonic and fetal development.
  • Posted in Journal of Biological Chemistry, Publications
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