Compounds that select against the tetracycline-resistance efflux pump

September 19th, 2016 by Laura K Stone

Nature Chemical Biology 12, 902 (2016). doi:10.1038/nchembio.2176

Authors: Laura K Stone, Michael Baym, Tami D Lieberman, Remy Chait, Jon Clardy & Roy Kishony

We developed a competition-based screening strategy to identify compounds that invert the selective advantage of antibiotic resistance. Using our assay, we screened over 19,000 compounds for the ability to select against the TetA tetracycline-resistance efflux pump in Escherichia coli and identified two hits, β-thujaplicin and disulfiram. Treating a tetracycline-resistant population with β-thujaplicin selects for loss of the resistance gene, enabling an effective second-phase treatment with doxycycline.

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