The Heme Biosynthesis Pathway is Essential for Plasmodium falciparum Development in Mosquito Stage but not in Blood Stages [Metabolism]

October 29th, 2014 by Ke, H., Sigala, P. A., Miura, K., Morrisey, J. M., Mather, M. W., Crowley, J. R., Henderson, J. P., Goldberg, D. E., Long, C. A., Vaidya, A. B.

Heme is an essential cofactor for aerobic organisms. Its redox chemistry is central to a variety of biological functions mediated by hemoproteins. In blood stages, malaria parasites consume most of the hemoglobin inside the infected erythrocytes, forming nontoxic hemozoin crystals from large quantities of heme released during digestion. At the same time, the parasites possess a heme de novo biosynthetic pathway. This pathway in the human malaria parasite Plasmodium falciparum has been considered essential and proposed as a potential drug target. However, we successfully disrupted the first and last genes of the pathway, individually and in combination. These knockout parasite lines, lacking δ-aminolevulinic acid synthase (ALAS) and/or ferrochelatase (FC), grew normally in blood-stage culture and exhibited no changes in sensitivity to heme-related antimalarial drugs. We developed a sensitive LC-MS/MS assay to monitor stable isotope incorporation into heme from its precursor 13C4-5-aminolevulinic acid (5-ALA), and this assay confirmed that de novo heme synthesis was ablated in FC knockout parasites. Disrupting the FC gene also caused no defects in gametocyte generation or maturation but resulted in a greater than 70% reduction in male gamete formation and completely prevented oocyst formation in female Anopheles stephensi mosquitoes. Our data demonstrate that the heme biosynthesis pathway is not essential for asexual blood-stage growth of P. falciparum parasites but is required for mosquito transmission. Drug inhibition of pathway activity is therefore unlikely to provide successful antimalarial therapy. These data also suggest the existence of a parasite mechanism for scavenging host heme to meet metabolic needs.