Protein engineering: Redirecting membrane machinery

August 18th, 2017 by Kalistyn Burley

Nature Chemical Biology 13, 927 (2017). doi:10.1038/nchembio.2451

Authors: Kalistyn Burley & Celia W Goulding

The ability to solubilize membrane proteins while retaining their native function is a persistent challenge. Re-engineering of the membrane protein DsbB into a soluble cytoplasmic version maintained its activity and enabled recompartmentalization of the periplasmic DsbAB disulfide bond–forming system.