12-O-tetradecanoylphorbol-13-acetate Promotes Breast Cancer Cell Motility by Increasing S100A14 Level in a Kruppel-like transcription factor 4 (KLF4)-dependent Manner [Gene Regulation]

February 14th, 2014 by He, H., Li, S., Chen, H., Li, L., Xu, C., Ding, F., Zhan, Y., Ma, J., Zhang, S., Shi, Y., Qu, C., Liu, Z.

The S100 protein family represents the largest subgroup of calcium binding EF-hand type proteins. These proteins have been reported to be involved in a wide range of biological functions that are related to normal cell development and tumorigenesis. S100A14 is a recently identified member of the S100 protein family and differentially expressed in a number of different human malignancies. However, the transcriptional regulation of S100A14 and its role in breast cancer needs to be further investigated. Here, we dissected that 12-O-tetradecanoylphorbol-13-acetate (TPA) upregulated the expression of KLF4 and facilitated its binding directly to two conserved GC-rich DNA segments within the S100A14 promoter, which is essential for the transactivation of KLF4 induced S100A14 expression. Furthermore, stable silencing of KLF4 significantly suppressed breast cancer cell migration induced by TPA. Collectively, these results offer insights into that TPA provokes cell motility through regulating the expression and function of S100A14 in a KLF4-dependent manner.
  • Posted in Journal of Biological Chemistry, Publications
  • Comments Off on 12-O-tetradecanoylphorbol-13-acetate Promotes Breast Cancer Cell Motility by Increasing S100A14 Level in a Kruppel-like transcription factor 4 (KLF4)-dependent Manner [Gene Regulation]