Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

January 29th, 2018 by Hao Yin

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity

Partial DNA-guided Cas9 enables genome editing with reduced off-target activity, Published online: 29 January 2018; doi:10.1038/nchembio.2559

Partial substitution of CRISPR RNAs with DNA nucleotides retains CRISPR–Cas9 genome editing activity while enhancing efficiency and specificity within cells, suggesting that DNA–RNA hybrids may be economical reagents for targeted genome editing.
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