Active turnover of genomic methylcytosine in pluripotent cells

August 10th, 2020 by Fabio Spada

Nature Chemical Biology, Published online: 10 August 2020; doi:10.1038/s41589-020-0621-y

The combination of heavy isotope labeling and ultra-high-pressure liquid chromatography coupled to triple-quadrupole mass spectrometry (UHPLC–MS) is used to quantify modified genomic cytosines in pluripotent stem cells in different states and reveals active turnover of methylcytidine in oxidation-dependent and oxidation-independent manners.