Protein kinase D2 assembles a multiprotein complex at the Trans-Golgi-network to regulate matrix metalloproteinase secretion [Signal Transduction]

October 27th, 2015 by Eiseler, T., Wille, C., Koehler, C., Illing, A., Seufferlein, T.

Vesicle formation and fission are tightly regulated at the Trans-Golgi-network (TGN) during constitutive secretion. Two major protein families regulate these processes: members of the Adenosyl-ribosylation-factor-family of small G-proteins (ARFs) and the Protein kinase D (PKD) family of serine/threonine kinases. The functional relationship between these two key regulators of protein transport from the TGN so far is elusive. We here demonstrate the assembly of a novel functional protein complex at the TGN and its key members: Cytosolic PKD2 binds ARL1 and shuttles ARL1 to the TGN. ARL1, in turn, localizes Arfaptin2 to the TGN. At the TGN, where PKD2 interacts with active ARF1, PKD2 and ARL1 are required for the assembly of a complex comprising of ARF1 and Arfaptin2 leading to secretion of MMP2 and -7. In conclusion, our data indicate that PKD2 is a core factor in the formation of this multiprotein complex at the TGN that controls constitutive secretion of MMP cargo.
  • Posted in Journal of Biological Chemistry, Publications
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