Histone H3K14 Acetylation Facilitates DNA Repair in a Positioned Nucleosome by Stabilizing the Binding of Chromatin Remodeler RSC [DNA and Chromosomes]

February 10th, 2014 by Duan, M.-R., Smerdon, M. J.

Histone H3 acetylation is induced by UV damage in yeast and may play an important role in regulating repair of UV photolesions in nucleosome-loaded genomic loci. However, it remains elusive how H3 acetylation facilitates repair. We generated a strongly positioned nucleosome containing homogeneously acetylated H3 at K14 (H3K14ac) and investigated possible mechanisms by which H3K14 acetylation modulates repair. We show that H3K14ac does not alter nucleosome unfolding dynamics, nor does it enhance repair of UV-induced cyclobutane pyrimidine dimers (CPDs) by UV photolyase. Importantly, however, nucleosomes with H3K14ac have a higher affinity for purified chromatin remodeling complex RSC (Remodels Structure of Chromatin) and show greater CPD repair compared to unacetylated nucleosomes. Our study indicates that, by anchoring RSC, H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage.
  • Posted in Journal of Biological Chemistry, Publications
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