The endoplasmic reticulum-based acetyltransferases, ATase1 and ATase2, associate with the oligosaccharyl-transferase to acetylate correctly folded polypeptides [Protein Structure and Folding]

October 9th, 2014 by Ding, Y., Dellisanti, C. D., Ko, M. H., Czajkowski, C., Puglielli, L.

The endoplasmic reticulum (ER) has two membrane-bound acetyltransferases responsible for the endoluminal Nϵ-lysine acetylation of ER-transiting and -resident proteins. Mutations that impair the ER-based acetylation machinery are associated with developmental defects and a familial form of spastic paraplegia. Deficient ER acetylation in the mouse leads to defects of the immune and nervous system. Here, we report that both ATase1 and ATase2 form homo- and hetero-dimers and associate with members of the oligosaccharyl-transferase (OST) complex. In contrast to the OST, the ATases only modify correctly folded polypetides. Collectively, our studies suggest that one of the functions of the ATases is to work in concert with the OST and select correctly folded from unfolded/misfolded transiting polypeptides.
  • Posted in Journal of Biological Chemistry, Publications
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