SLC17A9 functions as a lysosomal ATP transporter and regulates cell viability [Signal Transduction]

June 24th, 2014 by Cao, Q., Zhao, K., Zhong, X. Z., Zou, Y., Yu, H., Huang, P., Xu, T.-L., Dong, X.-P.

Lysosomes contain abundant ATP, which is released through lysosomal exocytosis following exposure to various stimuli. However, molecular mechanisms underlying lysosomal ATP accumulation remain unknown. VNUT (vesicular nucleotide transporter), also known as SLC17A9 (solute carrier family 17 member 9), has been shown to function in ATP transport across secretory vesicles/granules membrane in adrenal chromaffin cells, T cells, and pancreatic cells. Here, using mammalian cell lines we report that SLC17A9 is highly enriched in lysosomes and functions as an ATP transporter in those organelles. SLC17A9 deficiency reduced lysosome ATP accumulation and compromised lysosome function, resulting in cell death. Our data suggests that SLC17A9 activity mediates lysosomal ATP accumulation and plays an important role in lysosomal physiology and cell viability.