High Yield Non-Detergent Isolation of PS I-LHC II Membranes from Spinach Thylakoids: Implications for the Organization of the PS I Antennae in Higher Plants [Plant Biology]

June 8th, 2015 by Bell, A. J., Frankel, L. K., Bricker, T. M.

Styrene-maleic acid copolymer was used to effect a non-detergent partial solubilization of thylakoids from spinach. A high density membrane fraction, which was not solubilized by the copolymer, was isolated and was highly enriched in the PS I-LHC II supercomplex and depleted of PS II, the cytochrome b6/f complex and ATP Synthase. The LHC II associated with the supercomplex appeared to be energetically coupled to PS I based on 77 K fluorescence, P700 photooxidation and PS I electron transport light saturation experiments. The chl a/b ratio of the PS I-LHC II membranes was 3.2 ± 0.9, indicating that on average, three LHC II trimers may associate with each PS I. The implication of these findings within the context of higher plant PS I antenna organization is discussed.
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