The Polycomb Repressive Complex 1 Protein BMI1 is Required for Constitutive Heterochromatin Formation and Silencing in Mammalian Somatic Cells [Genomics and Proteomics]

October 14th, 2015 by Abdouh, M., Hanna, R., El Hajjar, J., Flamier, A., Bernier, G.

The Polycomb Repressive Complex 1 (PRC1), containing the core BMI1 and RING1A/B proteins, mono-ubiquitylates histone H2A (H2Aub) and is associated with silenced developmental genes at facultative heterochromatin. It is however assumed that the PRC1 is excluded from constitutive heterochromatin in somatic cells based on work performed on mouse embryonic stem cells and oocytes. We show here that BMI1 is required for constitutive heterochromatin formation and silencing in human and mouse somatic cells. BMI1 was highly enriched at intergenic and pericentric heterochromatin, co-immunoprecipitated with the architectural heterochromatin proteins HP1, DEK1 and ATRx, and was required for their localization. In contrast, BRCA1 localization was BMI1-independent and partially redundant with that of BMI1 for H2Aub deposition, constitutive heterochromatin formation and silencing. These observations suggest a dynamic and developmentally regulated model of PRC1 occupancy at constitutive heterochromatin, and where BMI1 function in somatic cells is to stabilize the repetitive genome
  • Posted in Journal of Biological Chemistry, Publications
  • Comments Off on The Polycomb Repressive Complex 1 Protein BMI1 is Required for Constitutive Heterochromatin Formation and Silencing in Mammalian Somatic Cells [Genomics and Proteomics]